HRP-conjugated Rabbit-Human IgE(Fc) PolymAb

Product Overview

• Article Name: HRP-conjugated Rabbit-Human IgE(Fc) PolymAb
• Biozol Catalog Number: ABB-AS161PM
• Supplier Catalog Number: AS161PM
• Alternative Catalog Number: ABB-AS161PM-100UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
• Conjugation: HRP
• Alternative Names: Igh2, IGHE, IGHEP1, Human IgE

Product Description

IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock

Product Properties

• Molecular Weight: 47kDa
• UniProt: P01854
• Purity: Affinity purification
• Target: IgE
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: WB,1:80000 - 1:170000|ELISA, 1:5000-1:10000
• Application Notes: Cross-Reactivity: Human, ResearchArea: Adaptive immunity, Immunity.

Images

Western blot analysis of various lysates using HRP-conjugated Rabbit-Human IgE(Fc) PolymAb (AS161PM) at 1:170000 dilutionincubated overnight at 4°C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 10ng µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): Human IgG1,Human IgG2,Human IgG3,Human IgG4,Human IgA1,Human IgD,Human IgM,Human IgE
Exposure time: 90s.