Figure-1: Intracellular flow analysis of Pax8 in Raji cells using 0.5 µg/10 6 cells of antibody (Clone: ABM5F33). Green represents isotype control, red represents anti-Pax8 antibody. Goat anti-mouse PE conjugate was used as secondary antibody. (Cells were fixed with 4% paraformaldehyde for 10 min and washed with PBS by centrifuging at 1100 for 5 min followed by permeabilization for 20 min and washed again as mentioned above. Then cell were incubated with primary antibody for 45 min. and after
Figure-2: Western blot analysis of PAX8. Anti-PAX8 antibody (Clone: ABM5F33) was used at 2 µg/ml on Ramos lysate.
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