Monoclonal Antibody to PAX8 (Clone:ABM5F33 ), Clone: [ABM5F33], Mouse

Product Overview

• Article Name: Monoclonal Antibody to PAX8 (Clone:ABM5F33 ), Clone: [ABM5F33], Mouse
• Biozol Catalog Number: ABI-10-7603
• Supplier Catalog Number: 10-7603
• Alternative Catalog Number: ABI-10-7603-100UG
• Manufacturer: Abeomics
• Host: Mouse
• Category: Antikörper
• Application: FACS, WB
• Species Reactivity: Human
• Immunogen: A partial length recombinant protein (a.a 28-261) of PAX8 was used as the immunogen for this antibody.
• Alternative Names: Paired box protein Pax-8

Product Properties

• Clonality: Monoclonal
• Clone Designation: [ABM5F33]
• NCBI: 7849
• UniProt: Q06710
• Purity: Protein G Chromatography
• Form: Purified
• Target: PAX8
• Antibody Type: Monoclonal Antibody

Application Notes

• Application Dilute: WB: 2-4 µg/ml, FACS: 0.5-1 µg/10 6

Images

Figure-1: Intracellular flow analysis of Pax8 in Raji cells using 0.5 µg/10 6 cells of antibody (Clone: ABM5F33). Green represents isotype control, red represents anti-Pax8 antibody. Goat anti-mouse PE conjugate was used as secondary antibody. (Cells were fixed with 4% paraformaldehyde for 10 min and washed with PBS by centrifuging at 1100 for 5 min followed by permeabilization for 20 min and washed again as mentioned above. Then cell were incubated with primary antibody for 45 min. and after

Figure-2: Western blot analysis of PAX8. Anti-PAX8 antibody (Clone: ABM5F33) was used at 2 µg/ml on Ramos lysate.