Human IL-1 Alpha (Interleukin 1 Alpha) Pre-Coated ELISA Kit
Artikelnummer:
ABI-90-2081
Hersteller Artikelnummer:
90-2081
Alternativnummer:
ABI-90-2081-1X96TESTS
Hersteller:
Abeomics
Kategorie:
Sonstiges
Applikation:
ELISA
Spezies Reaktivität:
Human
This assay employs an antibody specific for anti-human IL-1Alphacoated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1Alphapresent in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-humanIL-1Alphaantibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1Alphabound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Application Verdünnung:
This immunoassay kit allows for the in vitro quantitative determination of Human IL-1 Alpha concentrations in serum, plasma and other biological fluids. Detection Range: 3.9 - 250 pg/ml Sensitivity: < 2.34 pg/ml
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