Human IL-1 Alpha (Interleukin 1 Alpha) Pre-Coated ELISA Kit

Product Overview

• Article Name: Human IL-1 Alpha (Interleukin 1 Alpha) Pre-Coated ELISA Kit
• Biozol Catalog Number: ABI-90-2081
• Supplier Catalog Number: 90-2081
• Alternative Catalog Number: ABI-90-2081-1X96TESTS
• Manufacturer: Abeomics
• Category: Sonstiges
• Application: ELISA
• Species Reactivity: Human

Product Description

This assay employs an antibody specific for anti-human IL-1Alphacoated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1Alphapresent in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-humanIL-1Alphaantibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1Alphabound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Application Notes

• Application Dilute: This immunoassay kit allows for the in vitro quantitative determination of Human IL-1 Alpha concentrations in serum, plasma and other biological fluids. Detection Range: 3.9 - 250 pg/ml Sensitivity: < 2.34 pg/ml