HepG2 (human hepatoblastoma) whole cell lysate (denatured), Human
Artikelnummer:
ABN-L074V1
Hersteller Artikelnummer:
L074V1
Alternativnummer:
ABN-L074V1-200
Hersteller:
Abnova
Wirt:
Human
Kategorie:
Zellen/Zellkultur
Applikation:
WB
Spezies Reaktivität:
Human
Whole cell lysate (denatured).
Puffer:
In ready-to-use 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue).
Quelle:
Liver
Application Verdünnung:
The lysate is ready to load on SDS-PAGE for Western blotting.
Anwendungsbeschreibung:
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Mi
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