HepG2 (human hepatoblastoma) whole cell lysate (denatured), Human
Biozol Catalog Number:
ABN-L074V1
Supplier Catalog Number:
L074V1
Alternative Catalog Number:
ABN-L074V1-200
Manufacturer:
Abnova
Host:
Human
Category:
Zellen/Zellkultur
Application:
WB
Species Reactivity:
Human
Whole cell lysate (denatured).
Buffer:
In ready-to-use 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue).
Source:
Liver
Application Dilute:
The lysate is ready to load on SDS-PAGE for Western blotting.
Application Notes:
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Mi
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