| SARSCoV2 Spike Trimer, His Tag (BA.3/Omicron) (SPNC5225) is expressed from human 293 cells (HEK293). It contains AA Val 16 Pro 1213 (Accession QHD43416.1 (A67V,H69del,V70del,T95I,G142D,V143del,Y144del,Y145del,N211del,L212I,G339D,S371F,S373P,S375F,D405N,K417N,N440K,G446S,S477N,T478K,E484A,Q493R,Q498R,N501Y,Y505H,D614G,H655Y,N679K,P681H,N764K,D796Y,Q954H,N969K,R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P)). The spike mutations are identified on the SARSCoV2 Omicron variant (Pango lineage: BA.3, GISAID clade: GRA, Nextstrain clade: 21M). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the Cterminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARSCoV2 S protein and abolish the furin cleavage site, respectively. - Proteine/Peptide |