Desmin, Recombinant, Human, Liquid (DES, CMD1I, CSM1, CSM2, FLJ12025, FLJ39719, FLJ41013, FLJ41793, Intermediate Filament Protein)
Catalog Number:
USB-D3221-14B
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| Article Name: | Desmin, Recombinant, Human, Liquid (DES, CMD1I, CSM1, CSM2, FLJ12025, FLJ39719, FLJ41013, FLJ41793, Intermediate Filament Protein) |
| Biozol Catalog Number: | USB-D3221-14B |
| Supplier Catalog Number: | D3221-14B |
| Alternative Catalog Number: | USB-D3221-14B-50 |
| Manufacturer: | US Biological |
| Category: | Molekularbiologie |
| Application: | ELISA, WB |
| Desmin is the main intermediate filament in mature skeletal, cardiac and smooth-muscle cells. Can be used to distinguish rhabdomyosarcomas and leiomyosarcomas from other soft tissue tumors, lymphomas and carcinomas. Molecular Weight: Calculated from sequence: 53.5396kD Observed (SDS Gel Electrophoresis): 55kD Isoelectric Point: pI 5.16 (calculated from sequence). Applications: Suitable for use as protein standard in 1D and 2D SDS gel electrophoresis, in Immunoassays and in immunization. Other applications not tested. Reconstitution to Filaments: Protofilaments and filament complexes are obtained by dialyzing the reconstituted polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50mM sodium chloride, 2mM DTT, 10mM Tris-HCl, pH 7.4) For Use in Immunization: The solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbeccos PBS). Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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