Pax7 Antibody - middle region

Produktübersicht

• Artikelname: Pax7 Antibody - middle region
• Artikelnummer: ASB-ARP30947_P050
• Hersteller Artikelnummer: ARP30947_P050
• Alternativnummer: ASB-ARP30947_P050-25UL,ASB-ARP30947_P050-100UL
• Hersteller: Aviva
• Wirt: Rabbit
• Kategorie: Proteine/Peptide
• Applikation: IF, WB
• Spezies Reaktivität: Bovine, Canine, Goat, Guinea pig, Human, Mouse, Rabbit, Rat, Zebrafish
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human Pax7
• Alternative Synonym: RGD1564360

Produktbeschreibung

The function of Pax7 remains unknown.

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 0.5 mg/ml
• Molekulargewicht: 55 kDa
• NCBI: 500574
• Formulierung: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Bilder

Sample Type : C2C12

Immunofluorescence -- Sample Type: Overexpression of Pax7 in C2C12 cellsDilution: 1:100

WB Suggested Anti-Pax7 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Rat Muscle

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment.

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment.

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.

Pax7 antibody - middle region (ARP30947_P050) in Rat Muscle using Western Blot