Pax7 Antibody - middle region
Catalog Number:
ASB-ARP30947_P050
- Images (7)
| Article Name: | Pax7 Antibody - middle region |
| Biozol Catalog Number: | ASB-ARP30947_P050 |
| Supplier Catalog Number: | ARP30947_P050 |
| Alternative Catalog Number: | ASB-ARP30947_P050-25UL,ASB-ARP30947_P050-100UL |
| Manufacturer: | Aviva |
| Host: | Rabbit |
| Category: | Proteine/Peptide |
| Application: | IF, WB |
| Species Reactivity: | Bovine, Canine, Goat, Guinea pig, Human, Mouse, Rabbit, Rat, Zebrafish |
| Immunogen: | The immunogen is a synthetic peptide directed towards the middle region of human Pax7 |
| Alternative Names: | RGD1564360 |
| The function of Pax7 remains unknown. |
| Clonality: | Polyclonal |
| Concentration: | 0.5 mg/ml |
| Molecular Weight: | 55 kDa |
| NCBI: | 500574 |
| Form: | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
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Sample Type : C2C12 |
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Immunofluorescence -- Sample Type: Overexpression of Pax7 in C2C12 cellsDilution: 1:100 |
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WB Suggested Anti-Pax7 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:312500 Positive Control: Rat Muscle |
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25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. |
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25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. |
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Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown. |
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Pax7 antibody - middle region (ARP30947_P050) in Rat Muscle using Western Blot |
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