GATA3 Antibody - N-terminal region
Artikelnummer:
ASB-ARP31857_P050
- Bilder (8)
| Artikelname: | GATA3 Antibody - N-terminal region |
| Artikelnummer: | ASB-ARP31857_P050 |
| Hersteller Artikelnummer: | ARP31857_P050 |
| Alternativnummer: | ASB-ARP31857_P050-25UL,ASB-ARP31857_P050-100UL |
| Hersteller: | Aviva |
| Wirt: | Rabbit |
| Kategorie: | Proteine/Peptide |
| Applikation: | IHC, WB |
| Spezies Reaktivität: | Bovine, Canine, Equine, Guinea pig, Human, Mouse, Porcine, Rabbit, Rat, Sheep, Zebrafish |
| Immunogen: | The immunogen is a synthetic peptide directed towards the N terminal region of human GATA3 |
| Alternative Synonym: | HDR, HDRS |
| GATA3 is crucially involved in IL-5 gene transcription in human peripheral CD4-positive t cells. This gene is involved in growth control and the maintenance of the differentiated state in epithelial cells and may contribute to tumorigenesis in breast cancer. |
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Immunohistochemistry with Human kidney lysate tissue at an antibody concentration of 5.0ug/ml using anti-GATA3 antibody (ARP31857_P050) |
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WB Suggested Anti-GATA3 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:1562500 Positive Control: MCF7 cell lysate |
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Host: Rabbit Target Name: GATA3 Sample Type: MCF7 Antibody Dilution: 1.0ug/mlGATA3 is strongly supported by BioGPS gene expression data to be expressed in Human MCF7 cells |
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Host: Rabbit Target Name: GATA3 Sample Type: Human Fetal Heart Antibody Dilution: 1.0ug/ml |
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Host: Rabbit Target Name: GATA3 Sample Type: Human Fetal Lung Antibody Dilution: 1.0ug/ml |
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Host: Rabbit Target Name: GATA3 Sample Type: MCF7 Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration: 1ug/ml Peptide Concentration: 5.0 ug/ml Lysate Quantity: 25ug/lane/lane Gel Concentration: 12% |
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Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown. |
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GATA3 antibody - N-terminal region (ARP31857_P050) in Human MCF-7 using Western Blot |
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