GATA3 Antibody - N-terminal region

Product Overview

• Article Name: GATA3 Antibody - N-terminal region
• Biozol Catalog Number: ASB-ARP31857_P050
• Supplier Catalog Number: ARP31857_P050
• Alternative Catalog Number: ASB-ARP31857_P050-25UL,ASB-ARP31857_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IHC, WB
• Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Porcine, Rabbit, Rat, Sheep, Zebrafish
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GATA3
• Alternative Names: HDR, HDRS

Product Description

GATA3 is crucially involved in IL-5 gene transcription in human peripheral CD4-positive t cells. This gene is involved in growth control and the maintenance of the differentiated state in epithelial cells and may contribute to tumorigenesis in breast cancer.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 48 kDa
• NCBI: 2625
• UniProt: P23771
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

Immunohistochemistry with Human kidney lysate tissue at an antibody concentration of 5.0ug/ml using anti-GATA3 antibody (ARP31857_P050)

WB Suggested Anti-GATA3 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: MCF7 cell lysate

Host: Rabbit
Target Name: GATA3
Sample Type: MCF7
Antibody Dilution: 1.0ug/mlGATA3 is strongly supported by BioGPS gene expression data to be expressed in Human MCF7 cells

Host: Rabbit
Target Name: GATA3
Sample Type: Human Fetal Heart
Antibody Dilution: 1.0ug/ml

Host: Rabbit
Target Name: GATA3
Sample Type: Human Fetal Lung
Antibody Dilution: 1.0ug/ml

Host: Rabbit
Target Name: GATA3
Sample Type: MCF7
Lane A: Primary Antibody
Lane B: Primary Antibody + Blocking Peptide
Primary Antibody Concentration: 1ug/ml
Peptide Concentration: 5.0 ug/ml
Lysate Quantity: 25ug/lane/lane
Gel Concentration: 12%

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.

GATA3 antibody - N-terminal region (ARP31857_P050) in Human MCF-7 using Western Blot