ESRRB encodes a protein with similarity to the estrogen receptor. Its function is unknown, however, a similar protein in mouse plays an essential role in placental development. This gene encodes a protein with similarity to the estrogen receptor. Its function is unknown, however, a similar protein in mouse plays an essential role in placental development. Sequence Note: The sequence AF094517.1 is from a chimeric clone. Only the ESRRB sequence was propagated into this RefSeq record. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Lane 1: 25ug mouse ES cellsLane 2: 25ug mouse ES cells overexpressing FLAG EsrrB (mouse)Lane 3: 25ug chromatin fraction CV1 cellsLane 4: 25ug chromatin fraction CV1 cells overexpressing FLAG EsrrB (mouse)Lane 5: 25ug chromatin fraction CV1 cells overexpressing FLAG EsrrB-Deltacter (mouse) Primary Antibody Dilution: 1:500 Secondary Antibody: Anti-rabbit-HRP Secondary Antibody Dilution: 1:3000 Gene Name: ESRRB Submitted by: Domenico Maiorano, Institute of Human Genetics (IGH)
25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment. Isoforms of 48 and 63 kDa are observed in some samples. Recommended dilution for this antibody is 1-3 ug/mL.
25 ug of the indicated Human whole cell or tissue extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment. Multiple isoforms contain this peptide sequence from 63 kDa to 48 kDa.
ESRRB antibody - N-terminal region (ARP32260_P050) in Mouse ES, fraction CV1 using Western Blot
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