ESRRB Antibody - N-terminal region

Product Overview

• Article Name: ESRRB Antibody - N-terminal region
• Biozol Catalog Number: ASB-ARP32260_P050
• Supplier Catalog Number: ARP32260_P050
• Alternative Catalog Number: ASB-ARP32260_P050-25UL,ASB-ARP32260_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: WB
• Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ESRRB
• Alternative Names: ERR2, ERRb, ESRL2, NR3B2, DFNB35, ERRbeta2, ERR beta-2

Product Description

ESRRB encodes a protein with similarity to the estrogen receptor. Its function is unknown, however, a similar protein in mouse plays an essential role in placental development. This gene encodes a protein with similarity to the estrogen receptor. Its function is unknown, however, a similar protein in mouse plays an essential role in placental development. Sequence Note: The sequence AF094517.1 is from a chimeric clone. Only the ESRRB sequence was propagated into this RefSeq record. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 48 kDa
• NCBI: 2103
• UniProt: O95718
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

WB Suggested Anti-ESRRB Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: MCF7 cell lysate

Lane 1: 25ug mouse ES cellsLane 2: 25ug mouse ES cells overexpressing FLAG EsrrB (mouse)Lane 3: 25ug chromatin fraction CV1 cellsLane 4: 25ug chromatin fraction CV1 cells overexpressing FLAG EsrrB (mouse)Lane 5: 25ug chromatin fraction CV1 cells overexpressing FLAG EsrrB-Deltacter (mouse)
Primary Antibody Dilution:
1:500
Secondary Antibody:
Anti-rabbit-HRP
Secondary Antibody Dilution:
1:3000
Gene Name:
ESRRB
Submitted by:
Domenico Maiorano, Institute of Human Genetics (IGH)

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment. Isoforms of 48 and 63 kDa are observed in some samples. Recommended dilution for this antibody is 1-3 ug/mL.

25 ug of the indicated Human whole cell or tissue extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment. Multiple isoforms contain this peptide sequence from 63 kDa to 48 kDa.

ESRRB antibody - N-terminal region (ARP32260_P050) in Mouse ES, fraction CV1 using Western Blot