CAPN1 Antibody - middle region

Produktübersicht

• Artikelname: CAPN1 Antibody - middle region
• Artikelnummer: ASB-ARP60936_P050
• Hersteller Artikelnummer: ARP60936_P050
• Alternativnummer: ASB-ARP60936_P050-25UL,ASB-ARP60936_P050-100UL
• Hersteller: Aviva
• Wirt: Rabbit
• Kategorie: Proteine/Peptide
• Applikation: IHC, WB
• Spezies Reaktivität: Bovine, Equine, Goat, Human, Mouse, Porcine, Rabbit, Rat, Sheep, Zebrafish
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human CAPN1
• Alternative Synonym: CANP, muCL, CANP1, SPG76, CANPL1, muCANP

Produktbeschreibung

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene.

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 0.5 mg/ml
• Molekulargewicht: 82kDa
• NCBI: 823
• UniProt: P07384
• Formulierung: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Bilder

Rabbit Anti-CAPN1 Antibody
Catalog Number: ARP60936_P050
Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue
Observed Staining: Cytoplasmic
Primary Antibody Concentration: N/A
Other Working Concentrations: 1:600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec

CAPN1 antibody - middle region (ARP60936_P050) validated by WB using HT1080 cell lysate at 1ug/mL.

Primary dilution: 1ug/mL Secondary dilution: 1:2000Tested with Hut-78, K562, Raj, U937, and Indomethacin Treated Jurkat cells

Lanes:
1. 20 ug Jurkat cell lysate
2. 20 ug Hut-78 cell lysate
3. 20 ug K562 cell lysate
4. 20 ug Raj cell lysate
5. 20 ug U937 cell lysate
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Anti-rabbit HRP
Secondary Antibody Dilution:
1:2000
Gene Name:
CAPN1
Submitted by:
Ewelina Swiderek, Institute of Immunology and Experimental Therapy, Wroclaw, Poland

CAPN1 antibody - middle region (ARP60936_P050) in Human HT1080 using Western Blot