CAPN1 Antibody - middle region

Product Overview

• Article Name: CAPN1 Antibody - middle region
• Biozol Catalog Number: ASB-ARP60936_P050
• Supplier Catalog Number: ARP60936_P050
• Alternative Catalog Number: ASB-ARP60936_P050-25UL,ASB-ARP60936_P050-100UL
• Manufacturer: Aviva
• Host: Rabbit
• Category: Proteine/Peptide
• Application: IHC, WB
• Species Reactivity: Bovine, Equine, Goat, Human, Mouse, Porcine, Rabbit, Rat, Sheep, Zebrafish
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human CAPN1
• Alternative Names: CANP, muCL, CANP1, SPG76, CANPL1, muCANP

Product Description

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene.

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 82kDa
• NCBI: 823
• UniProt: P07384
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Images

Rabbit Anti-CAPN1 Antibody
Catalog Number: ARP60936_P050
Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue
Observed Staining: Cytoplasmic
Primary Antibody Concentration: N/A
Other Working Concentrations: 1:600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec

CAPN1 antibody - middle region (ARP60936_P050) validated by WB using HT1080 cell lysate at 1ug/mL.

Primary dilution: 1ug/mL Secondary dilution: 1:2000Tested with Hut-78, K562, Raj, U937, and Indomethacin Treated Jurkat cells

Lanes:
1. 20 ug Jurkat cell lysate
2. 20 ug Hut-78 cell lysate
3. 20 ug K562 cell lysate
4. 20 ug Raj cell lysate
5. 20 ug U937 cell lysate
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Anti-rabbit HRP
Secondary Antibody Dilution:
1:2000
Gene Name:
CAPN1
Submitted by:
Ewelina Swiderek, Institute of Immunology and Experimental Therapy, Wroclaw, Poland

CAPN1 antibody - middle region (ARP60936_P050) in Human HT1080 using Western Blot