The immunogen is a synthetic peptide directed towards the C-terminal region of Human OTUD1
Alternative Synonym:
DUBA7, OTDC1
Deubiquitinating enzymes are proteases that specifically cleave ubiquitin linkages, negating the action of ubiquitin ligases. DUBA7 belongs to a DUB subfamily characterized by an ovarian tumor (OTU) domain.
Host: Rabbit Target: OTUD1 Positive control (+): Human lung (LU) Negative control (-): Human brain (BR) Antibody concentration: 1ug/ml
Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.
OTUD1 Antibody - C-terminal region (ARP71333_P050) in Human HepG2 using Western Blot
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