OTUD1 Antibody - C-terminal region

Catalog Number: ASB-ARP71333_P050
Article Name: OTUD1 Antibody - C-terminal region
Biozol Catalog Number: ASB-ARP71333_P050
Supplier Catalog Number: ARP71333_P050
Alternative Catalog Number: ASB-ARP71333_P050-25UL,ASB-ARP71333_P050-100UL
Manufacturer: Aviva
Host: Rabbit
Category: Proteine/Peptide
Application: WB
Species Reactivity: Bovine, Canine, Equine, Human, Mouse, Rat
Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of Human OTUD1
Alternative Names: DUBA7, OTDC1
Deubiquitinating enzymes are proteases that specifically cleave ubiquitin linkages, negating the action of ubiquitin ligases. DUBA7 belongs to a DUB subfamily characterized by an ovarian tumor (OTU) domain.
Clonality: Polyclonal
Concentration: 0.5 mg/ml
Molecular Weight: 51 kDa
NCBI: 220213
UniProt: Q5VV17
Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment.
Host: Rabbit
Target Name: OTUD1
Sample Type: HepG2 Whole Cell lysates
Antibody Dilution: 1.0ug/ml
Host: Rabbit
Target: OTUD1
Positive control (+): Human lung (LU)
Negative control (-): Human brain (BR)
Antibody concentration: 1ug/ml
Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.
OTUD1 Antibody - C-terminal region (ARP71333_P050) in Human HepG2 using Western Blot