Abbkine CheKine(TM) Alanine Aminotransferase (ALT/GPT) Activity Colorimetric Assay Kit is designed for detecting ALT/GPT activity in the sample.
Tag:
ALT/GPT
Anwendungsbeschreibung:
The kit provides a simple method for detecting ALT/GPT activity in a variety of biological samples such as animal tissues, plant tissues, cell culture: adherent or suspension cells, serum, bacterial. In the assay, ALT catalyzes the transamination reaction of alpha-ketoglutarate and alanine at 37C and pH7.4 to produce glutamic acid and pyruvate, pyruvate can react with 2,4-dinitrophenylhydrazine to form Pyruvate phenylhydrazone, which appears brownish red under alkaline conditions and has a characteristic absorption peak at 505 nm. The rate of pyruvate phenylhydrazone increase at 505 nm can reflect ALT/GPT activity.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten