CheKine(TM) Micro Alanine Aminotransferase (ALT/GPT) Activity Assay Kit

Artikelnummer: ASC-KTB1410
Artikelname: CheKine(TM) Micro Alanine Aminotransferase (ALT/GPT) Activity Assay Kit
Artikelnummer: ASC-KTB1410
Hersteller Artikelnummer: KTB1410
Alternativnummer: ASC-KTB1410-96
Hersteller: Abbkine Scientific
Kategorie: Kits/Assays
Alternative Synonym: ALT, GPT, Alanine aminotransferase, glutamate pyruvic transaminase
Abbkine CheKine(TM) Alanine Aminotransferase (ALT/GPT) Activity Colorimetric Assay Kit is designed for detecting ALT/GPT activity in the sample.
Tag: ALT/GPT
Anwendungsbeschreibung: The kit provides a simple method for detecting ALT/GPT activity in a variety of biological samples such as animal tissues, plant tissues, cell culture: adherent or suspension cells, serum, bacterial. In the assay, ALT catalyzes the transamination reaction of alpha-ketoglutarate and alanine at 37C and pH7.4 to produce glutamic acid and pyruvate, pyruvate can react with 2,4-dinitrophenylhydrazine to form Pyruvate phenylhydrazone, which appears brownish red under alkaline conditions and has a characteristic absorption peak at 505 nm. The rate of pyruvate phenylhydrazone increase at 505 nm can reflect ALT/GPT activity.