CheKine(TM) Micro Alanine Aminotransferase (ALT/GPT) Activity Assay Kit

Product Overview

• Article Name: CheKine(TM) Micro Alanine Aminotransferase (ALT/GPT) Activity Assay Kit
• Biozol Catalog Number: ASC-KTB1410
• Supplier Catalog Number: KTB1410
• Alternative Catalog Number: ASC-KTB1410-96
• Manufacturer: Abbkine Scientific
• Category: Kits/Assays
• Alternative Names: ALT, GPT, Alanine aminotransferase, glutamate pyruvic transaminase

Product Description

Abbkine CheKine(TM) Alanine Aminotransferase (ALT/GPT) Activity Colorimetric Assay Kit is designed for detecting ALT/GPT activity in the sample.

Product Properties

• Tag: ALT/GPT

Application Notes

• Application Notes: The kit provides a simple method for detecting ALT/GPT activity in a variety of biological samples such as animal tissues, plant tissues, cell culture: adherent or suspension cells, serum, bacterial. In the assay, ALT catalyzes the transamination reaction of alpha-ketoglutarate and alanine at 37C and pH7.4 to produce glutamic acid and pyruvate, pyruvate can react with 2,4-dinitrophenylhydrazine to form Pyruvate phenylhydrazone, which appears brownish red under alkaline conditions and has a characteristic absorption peak at 505 nm. The rate of pyruvate phenylhydrazone increase at 505 nm can reflect ALT/GPT activity.