Rat Soluble receptor activator of nuclear factor-kB ligand (sRANKL) ELISA Kit

Artikelnummer: ASC-KTE100068
Artikelname: Rat Soluble receptor activator of nuclear factor-kB ligand (sRANKL) ELISA Kit
Artikelnummer: ASC-KTE100068
Hersteller Artikelnummer: KTE100068
Alternativnummer: ASC-KTE100068-48,ASC-KTE100068-96
Hersteller: Abbkine Scientific
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Rat
Alternative Synonym: TNFSF11, CD254, ODF, OPGL, OPTB2, RANKL, TRANCE, hRANKL2, sOdf, OTTHUMP00000178585, TNF-related activation-induced cytokine, osteoclast differentiation factor, osteoprotegerin ligand, receptor activator of nucle
This Rat Soluble receptor activator of nuclear factor-kB ligand (sRANKL) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFSF11.
Detektionsbereich: Please inquire
Sensitivitaet: Please inquire
Tag: TNFSF11
NCBI: 117516
UniProt: Q9ESE2
Proben: Cell culture supernatantsSerumPlasmaOther biological fluids
Anwendungsbeschreibung: This Rat Soluble receptor activator of nuclear factor-kB ligand (sRANKL) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFSF11 in samples. An antibody specific for TNFSF11 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNFSF11 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFSF11 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFSF11 bound in the initial step. The color development is stopped and the intensity of the color is measured.