Rat Soluble receptor activator of nuclear factor-kB ligand (sRANKL) ELISA Kit

Catalog Number: ASC-KTE100068
Article Name: Rat Soluble receptor activator of nuclear factor-kB ligand (sRANKL) ELISA Kit
Biozol Catalog Number: ASC-KTE100068
Supplier Catalog Number: KTE100068
Alternative Catalog Number: ASC-KTE100068-48,ASC-KTE100068-96
Manufacturer: Abbkine Scientific
Category: Kits/Assays
Application: ELISA
Species Reactivity: Rat
Alternative Names: TNFSF11, CD254, ODF, OPGL, OPTB2, RANKL, TRANCE, hRANKL2, sOdf, OTTHUMP00000178585, TNF-related activation-induced cytokine, osteoclast differentiation factor, osteoprotegerin ligand, receptor activator of nucle
This Rat Soluble receptor activator of nuclear factor-kB ligand (sRANKL) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFSF11.
Range: Please inquire
Sensitivity: Please inquire
Tag: TNFSF11
NCBI: 117516
UniProt: Q9ESE2
Samples: Cell culture supernatantsSerumPlasmaOther biological fluids
Application Notes: This Rat Soluble receptor activator of nuclear factor-kB ligand (sRANKL) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFSF11 in samples. An antibody specific for TNFSF11 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNFSF11 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFSF11 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFSF11 bound in the initial step. The color development is stopped and the intensity of the color is measured.