EliKine(TM) Human TNF-alpha ELISA Kit

Produktübersicht

• Artikelname: EliKine(TM) Human TNF-alpha ELISA Kit
• Artikelnummer: ASC-KTE6032
• Hersteller Artikelnummer: KTE6032
• Alternativnummer: ASC-KTE6032-48, ASC-KTE6032-96
• Hersteller: Abbkine Scientific
• Kategorie: Kits/Assays
• Applikation: ELISA
• Spezies Reaktivität: Human
• Alternative Synonym: TNF-alpha, TNF, DADB-70P7.1, DIF, TNF-alpha, TNFA, TNFSF2, APC1 protein, TNF superfamily, member 2, TNF, macrophage-derived, TNF, monocyte-derived, cachectin, tumor necrosis factor alpha, TNFSF7

Produktbeschreibung

EliKine(TM) Human TNF-alpha ELISA Kit employs a two-site sandwich ELISA to quantitate Human TNF-alpha.

Produkteigenschaften

• Detektionsbereich: 7.8 pg/ml-500 pg/ml
• Sensitivitaet: 4 pg/mL
• Tag: TNFA
• NCBI: 7124
• UniProt: P01375
• Proben: Cell culture supernatantsSerumPlasmaOther biological fluids

Anwendungsbeschreibung

• Anwendungsbeschreibung: EliKine(TM) Human TNF-alpha ELISA Kit employs a two-site sandwich ELISA to quantitate TNF-alpha in samples. An antibody specific for TNF-alpha has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNF-alpha present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNF-alpha is added to the wells. After washing, proprietary EliKine(TM) Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNF-alpha bound in the initial step. The color development is stopped and the intensity of the color is measured.