EliKine(TM) Human TNF-alpha ELISA Kit

Product Overview

• Article Name: EliKine(TM) Human TNF-alpha ELISA Kit
• Biozol Catalog Number: ASC-KTE6032
• Supplier Catalog Number: KTE6032
• Alternative Catalog Number: ASC-KTE6032-48, ASC-KTE6032-96
• Manufacturer: Abbkine Scientific
• Category: Kits/Assays
• Application: ELISA
• Species Reactivity: Human
• Alternative Names: TNF-alpha, TNF, DADB-70P7.1, DIF, TNF-alpha, TNFA, TNFSF2, APC1 protein, TNF superfamily, member 2, TNF, macrophage-derived, TNF, monocyte-derived, cachectin, tumor necrosis factor alpha, TNFSF7

Product Description

EliKine(TM) Human TNF-alpha ELISA Kit employs a two-site sandwich ELISA to quantitate Human TNF-alpha.

Product Properties

• Range: 7.8 pg/ml-500 pg/ml
• Sensitivity: 4 pg/mL
• Tag: TNFA
• NCBI: 7124
• UniProt: P01375
• Samples: Cell culture supernatantsSerumPlasmaOther biological fluids

Application Notes

• Application Notes: EliKine(TM) Human TNF-alpha ELISA Kit employs a two-site sandwich ELISA to quantitate TNF-alpha in samples. An antibody specific for TNF-alpha has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNF-alpha present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNF-alpha is added to the wells. After washing, proprietary EliKine(TM) Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNF-alpha bound in the initial step. The color development is stopped and the intensity of the color is measured.