Human NAD-dependent deacetylase sirtuin-2 (SIRT2/SIR2L/SIR2L2) ELISA Kit

Artikelnummer: ASC-KTE60653
Artikelname: Human NAD-dependent deacetylase sirtuin-2 (SIRT2/SIR2L/SIR2L2) ELISA Kit
Artikelnummer: ASC-KTE60653
Hersteller Artikelnummer: KTE60653
Alternativnummer: ASC-KTE60653-48, ASC-KTE60653-96
Hersteller: Abbkine Scientific
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Human
Alternative Synonym: SIRT2, SIR2, SIR2L, SIR2L2, silencing information regulator 2-like, silent information regulator 2, sir2-related protein type 2, sirtuin 2, sirtuin type 2
This Human NAD-dependent deacetylase sirtuin-2 (SIRT2/SIR2L/SIR2L2) ELISA Kit employs a two-site sandwich ELISA to quantitate SIRT2.
Detektionsbereich: Please inquire
Sensitivitaet: Please inquire
Tag: SIRT2
NCBI: 22933
UniProt: Q8IXJ6
Proben: Cell culture supernatantsSerumPlasmaOther biological fluids
Anwendungsbeschreibung: This Human NAD-dependent deacetylase sirtuin-2 (SIRT2/SIR2L/SIR2L2) ELISA Kit employs a two-site sandwich ELISA to quantitate SIRT2 in samples. An antibody specific for SIRT2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySIRT2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SIRT2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SIRT2 bound in the initial step. The color development is stopped and the intensity of the color is measured.