Human NAD-dependent deacetylase sirtuin-2 (SIRT2/SIR2L/SIR2L2) ELISA Kit

Product Overview

• Article Name: Human NAD-dependent deacetylase sirtuin-2 (SIRT2/SIR2L/SIR2L2) ELISA Kit
• Biozol Catalog Number: ASC-KTE60653
• Supplier Catalog Number: KTE60653
• Alternative Catalog Number: ASC-KTE60653-48, ASC-KTE60653-96
• Manufacturer: Abbkine Scientific
• Category: Kits/Assays
• Application: ELISA
• Species Reactivity: Human
• Alternative Names: SIRT2, SIR2, SIR2L, SIR2L2, silencing information regulator 2-like, silent information regulator 2, sir2-related protein type 2, sirtuin 2, sirtuin type 2

Product Description

This Human NAD-dependent deacetylase sirtuin-2 (SIRT2/SIR2L/SIR2L2) ELISA Kit employs a two-site sandwich ELISA to quantitate SIRT2.

Product Properties

• Range: Please inquire
• Sensitivity: Please inquire
• Tag: SIRT2
• NCBI: 22933
• UniProt: Q8IXJ6
• Samples: Cell culture supernatantsSerumPlasmaOther biological fluids

Application Notes

• Application Notes: This Human NAD-dependent deacetylase sirtuin-2 (SIRT2/SIR2L/SIR2L2) ELISA Kit employs a two-site sandwich ELISA to quantitate SIRT2 in samples. An antibody specific for SIRT2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySIRT2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SIRT2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SIRT2 bound in the initial step. The color development is stopped and the intensity of the color is measured.