Human Semaphorin-4A (SEMA4A) ELISA Kit

Artikelnummer: ASC-KTE60713
Artikelname: Human Semaphorin-4A (SEMA4A) ELISA Kit
Artikelnummer: ASC-KTE60713
Hersteller Artikelnummer: KTE60713
Alternativnummer: ASC-KTE60713-48, ASC-KTE60713-96
Hersteller: Abbkine Scientific
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Human
Alternative Synonym: SEMA4A, RP11-54H19.2, CORD10, FLJ12287, RP35, SEMAB, SEMB, OTTHUMP00000015917, OTTHUMP00000015918, sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, 4A, semaphori
This Human Semaphorin-4A (SEMA4A) ELISA Kit employs a two-site sandwich ELISA to quantitate SEMA4A.
Detektionsbereich: Please inquire
Sensitivitaet: Please inquire
Tag: SEMA4A
NCBI: 64218
UniProt: Q9H3S1
Proben: Cell culture supernatantsSerumPlasmaOther biological fluids
Anwendungsbeschreibung: This Human Semaphorin-4A (SEMA4A) ELISA Kit employs a two-site sandwich ELISA to quantitate SEMA4A in samples. An antibody specific for SEMA4A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySEMA4A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SEMA4A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SEMA4A bound in the initial step. The color development is stopped and the intensity of the color is measured.