Human Semaphorin-4A (SEMA4A) ELISA Kit

Catalog Number: ASC-KTE60713
Article Name: Human Semaphorin-4A (SEMA4A) ELISA Kit
Biozol Catalog Number: ASC-KTE60713
Supplier Catalog Number: KTE60713
Alternative Catalog Number: ASC-KTE60713-48, ASC-KTE60713-96
Manufacturer: Abbkine Scientific
Category: Kits/Assays
Application: ELISA
Species Reactivity: Human
Alternative Names: SEMA4A, RP11-54H19.2, CORD10, FLJ12287, RP35, SEMAB, SEMB, OTTHUMP00000015917, OTTHUMP00000015918, sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, 4A, semaphori
This Human Semaphorin-4A (SEMA4A) ELISA Kit employs a two-site sandwich ELISA to quantitate SEMA4A.
Range: Please inquire
Sensitivity: Please inquire
Tag: SEMA4A
NCBI: 64218
UniProt: Q9H3S1
Samples: Cell culture supernatantsSerumPlasmaOther biological fluids
Application Notes: This Human Semaphorin-4A (SEMA4A) ELISA Kit employs a two-site sandwich ELISA to quantitate SEMA4A in samples. An antibody specific for SEMA4A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySEMA4A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SEMA4A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SEMA4A bound in the initial step. The color development is stopped and the intensity of the color is measured.