Human Soluble protein-100 (S-100) ELISA Kit

Artikelnummer: ASC-KTE60771
Artikelname: Human Soluble protein-100 (S-100) ELISA Kit
Artikelnummer: ASC-KTE60771
Hersteller Artikelnummer: KTE60771
Alternativnummer: ASC-KTE60771-48, ASC-KTE60771-96
Hersteller: Abbkine Scientific
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Human
Alternative Synonym: S100A1, S100, S100-alpha, S100A, S100 alpha, S100 calcium-binding protein A1, S100 protein, alpha polypeptide
This Human Soluble protein-100 (S-100) ELISA Kit employs a two-site sandwich ELISA to quantitate S100A1.
Detektionsbereich: Please inquire
Sensitivitaet: Please inquire
Tag: S100A1
NCBI: 6271
UniProt: P23297
Proben: Cell culture supernatantsSerumPlasmaOther biological fluids
Anwendungsbeschreibung: This Human Soluble protein-100 (S-100) ELISA Kit employs a two-site sandwich ELISA to quantitate S100A1 in samples. An antibody specific for S100A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyS100A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for S100A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of S100A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.