Human Soluble protein-100 (S-100) ELISA Kit

Catalog Number: ASC-KTE60771
Article Name: Human Soluble protein-100 (S-100) ELISA Kit
Biozol Catalog Number: ASC-KTE60771
Supplier Catalog Number: KTE60771
Alternative Catalog Number: ASC-KTE60771-48, ASC-KTE60771-96
Manufacturer: Abbkine Scientific
Category: Kits/Assays
Application: ELISA
Species Reactivity: Human
Alternative Names: S100A1, S100, S100-alpha, S100A, S100 alpha, S100 calcium-binding protein A1, S100 protein, alpha polypeptide
This Human Soluble protein-100 (S-100) ELISA Kit employs a two-site sandwich ELISA to quantitate S100A1.
Range: Please inquire
Sensitivity: Please inquire
Tag: S100A1
NCBI: 6271
UniProt: P23297
Samples: Cell culture supernatantsSerumPlasmaOther biological fluids
Application Notes: This Human Soluble protein-100 (S-100) ELISA Kit employs a two-site sandwich ELISA to quantitate S100A1 in samples. An antibody specific for S100A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyS100A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for S100A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of S100A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.