Human A disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS5) ELISA Kit

Artikelnummer: ASC-KTE60921
Artikelname: Human A disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS5) ELISA Kit
Artikelnummer: ASC-KTE60921
Hersteller Artikelnummer: KTE60921
Alternativnummer: ASC-KTE60921-48, ASC-KTE60921-96
Hersteller: Abbkine Scientific
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Human
Alternative Synonym: ADAMTS5, ADAMTS11, ADMP-2, FLJ36738, a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase-2), aggrecanase-2
This Human A disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS5) ELISA Kit employs a two-site sandwich ELISA to quantitate ADAMTS5.
Detektionsbereich: Please inquire
Sensitivitaet: Please inquire
Tag: ADAMTS5
UniProt: Q9TT92
Proben: Cell culture supernatantsSerumPlasmaOther biological fluids
Anwendungsbeschreibung: This Human A disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS5) ELISA Kit employs a two-site sandwich ELISA to quantitate ADAMTS5 in samples. An antibody specific for ADAMTS5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADAMTS5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAMTS5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAMTS5 bound in the initial step. The color development is stopped and the intensity of the color is measured.