Human A disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS5) ELISA Kit

Product Overview

• Article Name: Human A disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS5) ELISA Kit
• Biozol Catalog Number: ASC-KTE60921
• Supplier Catalog Number: KTE60921
• Alternative Catalog Number: ASC-KTE60921-48, ASC-KTE60921-96
• Manufacturer: Abbkine Scientific
• Category: Kits/Assays
• Application: ELISA
• Species Reactivity: Human
• Alternative Names: ADAMTS5, ADAMTS11, ADMP-2, FLJ36738, a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase-2), aggrecanase-2

Product Description

This Human A disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS5) ELISA Kit employs a two-site sandwich ELISA to quantitate ADAMTS5.

Product Properties

• Range: Please inquire
• Sensitivity: Please inquire
• Tag: ADAMTS5
• UniProt: Q9TT92
• Samples: Cell culture supernatantsSerumPlasmaOther biological fluids

Application Notes

• Application Notes: This Human A disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS5) ELISA Kit employs a two-site sandwich ELISA to quantitate ADAMTS5 in samples. An antibody specific for ADAMTS5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADAMTS5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAMTS5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAMTS5 bound in the initial step. The color development is stopped and the intensity of the color is measured.