Human NEDD8-activating enzyme E1 regulatory subunit (NAE1) ELISA Kit

Artikelnummer: ASC-KTE61391
Artikelname: Human NEDD8-activating enzyme E1 regulatory subunit (NAE1) ELISA Kit
Artikelnummer: ASC-KTE61391
Hersteller Artikelnummer: KTE61391
Alternativnummer: ASC-KTE61391-48, ASC-KTE61391-96
Hersteller: Abbkine Scientific
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Human
Alternative Synonym: NAE1, A-116A10.1, APPBP1, HPP1, ula-1, NEDD8-activating enzyme E1 subunit, amyloid beta precursor protein binding protein 1, 59kDa, amyloid beta precursor protein-binding protein 1, 59kD, amyloid protein-bin
This Human NEDD8-activating enzyme E1 regulatory subunit (NAE1) ELISA Kit employs a two-site sandwich ELISA to quantitate NAE1.
Detektionsbereich: Please inquire
Sensitivitaet: Please inquire
Tag: NAE1
NCBI: 8883
UniProt: Q13564
Proben: Cell culture supernatantsSerumPlasmaOther biological fluids
Anwendungsbeschreibung: This Human NEDD8-activating enzyme E1 regulatory subunit (NAE1) ELISA Kit employs a two-site sandwich ELISA to quantitate NAE1 in samples. An antibody specific for NAE1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNAE1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NAE1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NAE1 bound in the initial step. The color development is stopped and the intensity of the color is measured.