Human NEDD8-activating enzyme E1 regulatory subunit (NAE1) ELISA Kit

Product Overview

• Article Name: Human NEDD8-activating enzyme E1 regulatory subunit (NAE1) ELISA Kit
• Biozol Catalog Number: ASC-KTE61391
• Supplier Catalog Number: KTE61391
• Alternative Catalog Number: ASC-KTE61391-48, ASC-KTE61391-96
• Manufacturer: Abbkine Scientific
• Category: Kits/Assays
• Application: ELISA
• Species Reactivity: Human
• Alternative Names: NAE1, A-116A10.1, APPBP1, HPP1, ula-1, NEDD8-activating enzyme E1 subunit, amyloid beta precursor protein binding protein 1, 59kDa, amyloid beta precursor protein-binding protein 1, 59kD, amyloid protein-bin

Product Description

This Human NEDD8-activating enzyme E1 regulatory subunit (NAE1) ELISA Kit employs a two-site sandwich ELISA to quantitate NAE1.

Product Properties

• Range: Please inquire
• Sensitivity: Please inquire
• Tag: NAE1
• NCBI: 8883
• UniProt: Q13564
• Samples: Cell culture supernatantsSerumPlasmaOther biological fluids

Application Notes

• Application Notes: This Human NEDD8-activating enzyme E1 regulatory subunit (NAE1) ELISA Kit employs a two-site sandwich ELISA to quantitate NAE1 in samples. An antibody specific for NAE1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNAE1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NAE1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NAE1 bound in the initial step. The color development is stopped and the intensity of the color is measured.