Human Mediator of RNA polymerase II transcription subunit 12 (MED12) ELISA Kit

Artikelnummer: ASC-KTE61671
Artikelname: Human Mediator of RNA polymerase II transcription subunit 12 (MED12) ELISA Kit
Artikelnummer: ASC-KTE61671
Hersteller Artikelnummer: KTE61671
Alternativnummer: ASC-KTE61671-48, ASC-KTE61671-96
Hersteller: Abbkine Scientific
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Human
Alternative Synonym: MED12, ARC240, CAGH45, FGS1, HOPA, KIAA0192, OKS, OPA1, TNRC11, TRAP230, CAG repeat protein 45, OPA-containing protein, activator-recruited cofactor 240 kDa component, human opposite paired, mediator of RNA po
This Human Mediator of RNA polymerase II transcription subunit 12 (MED12) ELISA Kit employs a two-site sandwich ELISA to quantitate MED12.
Detektionsbereich: Please inquire
Sensitivitaet: Please inquire
Tag: MED12
NCBI: 9968
UniProt: Q93074
Proben: Cell culture supernatantsSerumPlasmaOther biological fluids
Anwendungsbeschreibung: This Human Mediator of RNA polymerase II transcription subunit 12 (MED12) ELISA Kit employs a two-site sandwich ELISA to quantitate MED12 in samples. An antibody specific for MED12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMED12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MED12 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MED12 bound in the initial step. The color development is stopped and the intensity of the color is measured.