Human Mediator of RNA polymerase II transcription subunit 12 (MED12) ELISA Kit

Product Overview

• Article Name: Human Mediator of RNA polymerase II transcription subunit 12 (MED12) ELISA Kit
• Biozol Catalog Number: ASC-KTE61671
• Supplier Catalog Number: KTE61671
• Alternative Catalog Number: ASC-KTE61671-48, ASC-KTE61671-96
• Manufacturer: Abbkine Scientific
• Category: Kits/Assays
• Application: ELISA
• Species Reactivity: Human
• Alternative Names: MED12, ARC240, CAGH45, FGS1, HOPA, KIAA0192, OKS, OPA1, TNRC11, TRAP230, CAG repeat protein 45, OPA-containing protein, activator-recruited cofactor 240 kDa component, human opposite paired, mediator of RNA po

Product Description

This Human Mediator of RNA polymerase II transcription subunit 12 (MED12) ELISA Kit employs a two-site sandwich ELISA to quantitate MED12.

Product Properties

• Range: Please inquire
• Sensitivity: Please inquire
• Tag: MED12
• NCBI: 9968
• UniProt: Q93074
• Samples: Cell culture supernatantsSerumPlasmaOther biological fluids

Application Notes

• Application Notes: This Human Mediator of RNA polymerase II transcription subunit 12 (MED12) ELISA Kit employs a two-site sandwich ELISA to quantitate MED12 in samples. An antibody specific for MED12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMED12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MED12 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MED12 bound in the initial step. The color development is stopped and the intensity of the color is measured.