EliKine(TM) Mouse IL-1beta ELISA Kit

Artikelnummer: ASC-KTE7005
Artikelname: EliKine(TM) Mouse IL-1beta ELISA Kit
Artikelnummer: ASC-KTE7005
Hersteller Artikelnummer: KTE7005
Alternativnummer: ASC-KTE7005-48, ASC-KTE7005-96
Hersteller: Abbkine Scientific
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Mouse
Alternative Synonym: IL1B, IL-1, IL1-BETA, IL1F2, catabolin, preinterleukin 1 beta, pro-interleukin-1-beta
EliKine(TM) Mouse IL-1beta ELISA Kit employs a two-site sandwich ELISA to quantitate Mouse IL-1beta.
Detektionsbereich: 15.63 pg/ml-1000 pg/ml
Sensitivitaet: 8 pg/mL
Tag: IL1B
NCBI: 16176
UniProt: P10749
Proben: Cell culture supernatantsSerumPlasmaOther biological fluids
Anwendungsbeschreibung: EliKine(TM) Mouse IL-1beta ELISA Kit employs a two-site sandwich ELISA to quantitate IL-1beta in samples. An antibody specific for IL-1beta has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-1beta present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-1beta is added to the wells. After washing, proprietary EliKine(TM) Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-1beta bound in the initial step. The color development is stopped and the intensity of the color is measured.