EliKine(TM) Mouse IL-1beta ELISA Kit

Product Overview

• Article Name: EliKine(TM) Mouse IL-1beta ELISA Kit
• Biozol Catalog Number: ASC-KTE7005
• Supplier Catalog Number: KTE7005
• Alternative Catalog Number: ASC-KTE7005-48, ASC-KTE7005-96
• Manufacturer: Abbkine Scientific
• Category: Kits/Assays
• Application: ELISA
• Species Reactivity: Mouse
• Alternative Names: IL1B, IL-1, IL1-BETA, IL1F2, catabolin, preinterleukin 1 beta, pro-interleukin-1-beta

Product Description

EliKine(TM) Mouse IL-1beta ELISA Kit employs a two-site sandwich ELISA to quantitate Mouse IL-1beta.

Product Properties

• Range: 15.63 pg/ml-1000 pg/ml
• Sensitivity: 8 pg/mL
• Tag: IL1B
• NCBI: 16176
• UniProt: P10749
• Samples: Cell culture supernatantsSerumPlasmaOther biological fluids

Application Notes

• Application Notes: EliKine(TM) Mouse IL-1beta ELISA Kit employs a two-site sandwich ELISA to quantitate IL-1beta in samples. An antibody specific for IL-1beta has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-1beta present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-1beta is added to the wells. After washing, proprietary EliKine(TM) Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-1beta bound in the initial step. The color development is stopped and the intensity of the color is measured.