Anti-VIM Antibody , IgG1, Clone: [CL0157], Unconjugated, Mouse, Monoclonal

Artikelnummer: ATA-AMAB90516
Artikelname: Anti-VIM Antibody , IgG1, Clone: [CL0157], Unconjugated, Mouse, Monoclonal
Artikelnummer: ATA-AMAB90516
Hersteller Artikelnummer: AMAb90516
Alternativnummer: ATA-AMAB90516-100,ATA-AMAB90516-25
Hersteller: Atlas Antibodies
Wirt: Mouse
Kategorie: Antikörper
Applikation: IHC, WB
Spezies Reaktivität: Human
Immunogen: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Konjugation: Unconjugated
Alternative Synonym: Pan-Cancer
vimentin
Anti-VIM
Klonalität: Monoclonal
Konzentration: 0.1
Klon-Bezeichnung: [CL0157]
Isotyp: IgG1
NCBI: 7431
UniProt: P08670
Puffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Reinheit: Protein A purified
Sequenz: FANYIDKVRFLEQQNKILLAELEQLKGQGKSRLGDLYEEEMRELRRQVDQLTNDKARVEVERDNLAEDIMRLREKLQEEMLQREEAENTLQSFRQDVDNASLARLDLERKVES
Lagerung: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Target-Kategorie: VIM
Antibody Type: Monoclonal Antibody
Application Verdünnung: IHC: 1:2500 - 1:5000, WB: 1 µg/ml
Immunohistochemical staining of human rectum shows strong positivity in mesenchymal and lymphoid cells.
Immunohistochemical staining of human prostate shows strong immunoreactivity in the connective tissue.
Immunohistochemical staining of human cerebellum shows immunoreactivity in the endothelial cells.
Immunohistochemical staining of human kidney shows strong immunoreactivity in renal glomeruli but not tubuli cells.
Immunohistochemical staining of human skin shows distinct positivity in fibroblasts.
Western blot analysis in human cell lines U-251MG and MCF-7 using Anti-VIM antibody. Corresponding VIM RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
Lane 1: Marker [kDa]
Lane 2: Human tonsil tissue lysate
Lane 1: Marker [kDa]
Lane 2: Human cell line HeLa cytoplasmic fraction
Lane 3: Human cell line HeLa membrane fraction
Lane 4: Human cell line HeLa nuclear fraction
Lane 5: Human cell line HeLa chromatin fraction
Lane 6: Human cell line HeLa cytoskeletal fraction
AMAb90516
AMAb90516
AMAb90516