Anti-VIM Antibody , IgG1, Clone: [CL0157], Unconjugated, Mouse, Monoclonal

Catalog Number: ATA-AMAB90516
Article Name: Anti-VIM Antibody , IgG1, Clone: [CL0157], Unconjugated, Mouse, Monoclonal
Biozol Catalog Number: ATA-AMAB90516
Supplier Catalog Number: AMAb90516
Alternative Catalog Number: ATA-AMAB90516-100,ATA-AMAB90516-25
Manufacturer: Atlas Antibodies
Host: Mouse
Category: Antikörper
Application: IHC, WB
Species Reactivity: Human
Immunogen: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Conjugation: Unconjugated
Alternative Names: Pan-Cancer
vimentin
Anti-VIM
Clonality: Monoclonal
Concentration: 0.1
Clone Designation: [CL0157]
Isotype: IgG1
NCBI: 7431
UniProt: P08670
Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Purity: Protein A purified
Sequence: FANYIDKVRFLEQQNKILLAELEQLKGQGKSRLGDLYEEEMRELRRQVDQLTNDKARVEVERDNLAEDIMRLREKLQEEMLQREEAENTLQSFRQDVDNASLARLDLERKVES
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Target: VIM
Antibody Type: Monoclonal Antibody
Application Dilute: IHC: 1:2500 - 1:5000, WB: 1 µg/ml
Immunohistochemical staining of human rectum shows strong positivity in mesenchymal and lymphoid cells.
Immunohistochemical staining of human prostate shows strong immunoreactivity in the connective tissue.
Immunohistochemical staining of human cerebellum shows immunoreactivity in the endothelial cells.
Immunohistochemical staining of human kidney shows strong immunoreactivity in renal glomeruli but not tubuli cells.
Immunohistochemical staining of human skin shows distinct positivity in fibroblasts.
Western blot analysis in human cell lines U-251MG and MCF-7 using Anti-VIM antibody. Corresponding VIM RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
Lane 1: Marker [kDa]
Lane 2: Human tonsil tissue lysate
Lane 1: Marker [kDa]
Lane 2: Human cell line HeLa cytoplasmic fraction
Lane 3: Human cell line HeLa membrane fraction
Lane 4: Human cell line HeLa nuclear fraction
Lane 5: Human cell line HeLa chromatin fraction
Lane 6: Human cell line HeLa cytoskeletal fraction
AMAb90516
AMAb90516
AMAb90516