Anti-LEF1

Produktübersicht

• Artikelname: Anti-LEF1
• Artikelnummer: ATA-HPA002087
• Hersteller Artikelnummer: HPA002087
• Alternativnummer: ATA-HPA002087-100,ATA-HPA002087-25
• Hersteller: Atlas Antibodies
• Wirt: Rabbit
• Kategorie: Sonstiges
• Applikation: ChIP, ICC, IHC, WB
• Spezies Reaktivität: Human
• Immunogen: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
• Konjugation: Unconjugated
• Alternative Synonym: TCF10, TCF1ALPHA, TCF7L3

Produktbeschreibung

lymphoid enhancer-binding factor 1

Zusatzinformationen

Anti-LEF1

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 0.1 mg/ml
• Isotyp: IgG
• NCBI: 51176
• UniProt: Q9UJU2
• Puffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
• Reinheit: Affinity purified using the PrEST antigen as affinity ligand
• Sequenz: SDVNSKQGMSRHPPAPDIPTFYPLSPGGVGQITPPLGWQGQPVYPITGGFRQPYPSSLSVDTSMSRFSHHMIPGPPGPHTTGIPHPAIVTPQVKQEHPHTDSDLMHVKPQHEQRKEQEPKRPHIKKPLNAFMLY
• Lagerung: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
• Target-Kategorie: LEF1
• Antibody Type: Monoclonal Antibody

Anwendungsbeschreibung

• Application Verdünnung: ICC-IF: 0.25-2 µg/ml, IHC: 1:50 - 1:200, WB: 0.04-0.4 µg/ml

Bilder

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.

Immunohistochemical staining of human oral mucosa shows moderate nuclear positivity in subset of squamous epithelial cells.

Immunohistochemical staining of human lymph node shows moderate to strong nuclear positivity in non - germinal center cells.

Western blot analysis in human cell lines SK-MEL-30 and A-549 using Anti-LEF1 antibody. Corresponding LEF1 RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.

Western blot analysis in control (vector only transfected HEK293T lysate) and LEF1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402529).

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HPA002087-100ul