Anti-PRMT5 Antibody , Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: Anti-PRMT5 Antibody , Unconjugated, Rabbit, Polyclonal
• Artikelnummer: ATA-HPA005525
• Hersteller Artikelnummer: HPA005525
• Alternativnummer: ATA-HPA005525-100,ATA-HPA005525-25
• Hersteller: Atlas Antibodies
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ICC, IHC, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
• Konjugation: Unconjugated
• Alternative Synonym: HRMT1L5, SKB1, SKB1Hs

Produktbeschreibung

protein arginine methyltransferase 5

Zusatzinformationen

Anti-PRMT5

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 0.1 mg/ml
• Isotyp: IgG
• NCBI: 10419
• UniProt: O14744
• Puffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
• Reinheit: Affinity purified using the PrEST antigen as affinity ligand
• Sequenz: LLDRVPEEEKDTNVQVLMVLGAGRGPLVNASLRAAKQADRRIKLYAVEKNPNAVVTLENWQFEEWGSQVTVVSSDMREWVAPEKADIIVSELLGSFADNELSPECLDGAQHF
• Lagerung: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
• Target-Kategorie: PRMT5
• Antibody Type: Monoclonal Antibody

Anwendungsbeschreibung

• Application Verdünnung: ICC-IF: 0.25-2 µg/ml, IHC: 1:50 - 1:200, WB: 0.04-0.4 µg/ml

Bilder

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus & cytosol.

Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferus ducts.

Western blot analysis in Hep-G2 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PRMT5 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Western blot analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

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