Anti-PRKCA

Artikelnummer: ATA-HPA006563
Artikelname: Anti-PRKCA
Artikelnummer: ATA-HPA006563
Hersteller Artikelnummer: HPA006563
Alternativnummer: ATA-HPA006563-100
Hersteller: Atlas Antibodies
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ICC, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Konjugation: Unconjugated
Alternative Synonym: PKCA
protein kinase C, alpha
Anti-PRKCA
Klonalität: Polyclonal
Konzentration: 0.05 mg/ml
Isotyp: IgG
NCBI: 5578
UniProt: P17252
Puffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Reinheit: Affinity purified using the PrEST antigen as affinity ligand
Sequenz: CVINVPSLCGMDHTEKRGRIYLKAEVADEKLHVTVRDAKNLIPMDPNGLSDPYVKLKLIPDPKNESKQKTKTIRST
Lagerung: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Target-Kategorie: PRKCA
Antibody Type: Monoclonal Antibody
Application Verdünnung: ICC-IF: 0.25-2 µg/ml, IHC: 1:50 - 1:200, WB: 0.04-0.4 µg/ml
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.
Western blot analysis in human cell lines A-549 and Caco-2 using Anti-PRKCA antibody. Corresponding PRKCA RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
Western blot analysis using Anti-PRKCA antibody HPA006563 (A) shows similar pattern to independent antibody HPA006564 (B).
Western blot analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
HPA006563-100ul
HPA006563-100ul
HPA006563-100ul