Anti-HSPB8 Antibody , Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: Anti-HSPB8 Antibody , Unconjugated, Rabbit, Polyclonal
• Artikelnummer: ATA-HPA015876
• Hersteller Artikelnummer: HPA015876
• Alternativnummer: ATA-HPA015876-100,ATA-HPA015876-25
• Hersteller: Atlas Antibodies
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ICC, IHC, WB
• Spezies Reaktivität: Human
• Immunogen: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
• Konjugation: Unconjugated
• Alternative Synonym: E2IG1, H11, HSP22, HspB8

Produktbeschreibung

heat shock 22kDa protein 8

Zusatzinformationen

Anti-HSPB8

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 0.1 mg/ml
• Isotyp: IgG
• NCBI: 26353
• UniProt: Q9UJY1
• Puffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
• Reinheit: Affinity purified using the PrEST antigen as affinity ligand
• Sequenz: LLDDGFGMDPFPDDLTASWPDWALPRLSSAWPGTLRSGMVPRGPTATARFGVPAEGRTPPPFPGEPWKVCVNVHSFKPEELMVKTKDGYVEVSGKHEEKQQEGGIVSKNFTKKIQLP
• Lagerung: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
• Target-Kategorie: HSPB8
• Antibody Type: Monoclonal Antibody

Anwendungsbeschreibung

• Application Verdünnung: ICC-IF: 0.25-2 µg/ml, IHC: 1:20 - 1:50, WB: 0.04-0.4 µg/ml

Bilder

Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.

Immunohistochemical staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.

Western blot analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-HSPB8 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Western blot analysis in control (vector only transfected HEK293T lysate) and HSPB8 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY415329).

HPA015876

HPA015876

HPA015876