8-Azido-ATP can be incorporated into RNA by 3'-end labeling with Poly(A) Polymerase. Modified mRNA is ready for labeling with alkyne- or DBCO containing reaction partners. The possibility of performing strain-promoted Azide-Alkyne chemistry (SPAAC) enables the copper free click labeling of RNA at the nucleobases. Additionally 8-Azido-ATP can be used to study the ATP binding and hydrolysis in various ABC transporter whether via click chemistry or by exposure to UV light. The last one turns the azide into an a reactive nitrene that binds covalently to the protein it is bond to.
Konzentration:
10 mM
Molekulargewicht:
548.19 g/mol (free acid)
Reinheit:
95% (HPLC)
Formulierung:
Liquid
CAS Nummer:
[53696-59-6]
Formel:
C10H17N8O13P3
Anwendungsbeschreibung:
Triphosphate for modifying of 3END RNA
8-Azido-ATP
8-Azido-ATP
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