8-Azido-ATP can be incorporated into RNA by 3'-end labeling with Poly(A) Polymerase. Modified mRNA is ready for labeling with alkyne- or DBCO containing reaction partners. The possibility of performing strain-promoted Azide-Alkyne chemistry (SPAAC) enables the copper free click labeling of RNA at the nucleobases. Additionally 8-Azido-ATP can be used to study the ATP binding and hydrolysis in various ABC transporter whether via click chemistry or by exposure to UV light. The last one turns the azide into an a reactive nitrene that binds covalently to the protein it is bond to.
Concentration:
10 mM
Molecular Weight:
548.19 g/mol (free acid)
Purity:
95% (HPLC)
Form:
Liquid
CAS Number:
[53696-59-6]
Formula:
C10H17N8O13P3
Application Notes:
Triphosphate for modifying of 3END RNA
8-Azido-ATP
8-Azido-ATP
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