Anti-TMPRSS2 Antibody, Rabbit, Polyclonal

Artikelnummer: BOB-A00666
Artikelname: Anti-TMPRSS2 Antibody, Rabbit, Polyclonal
Artikelnummer: BOB-A00666
Hersteller Artikelnummer: A00666
Alternativnummer: BOB-A00666-100UL
Hersteller: Boster Bio
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ICC, IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: A synthetic peptide of human TMPRSS2(NP_005647.3).
Alternative Synonym: Transmembrane protease serine 2,3.4.21.-,Serine protease 10,Transmembrane protease serine 2 non-catalytic chain,Transmembrane protease serine 2 catalytic chain,TMPRSS2,PRSS10,
Boster Bio Anti-TMPRSS2 Antibody catalog A00666. Tested in WB,IHC,ICC/IF applications. This antibody reacts with Human,Mouse,Rat.
Klonalität: Polyclonal
Konzentration: 1 mg/ml
Molekulargewicht: 53859 MW
UniProt: O15393
Puffer: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Formulierung: Liquid
Application Verdünnung: WB: 1:500-1:2000IHC: 1:50-1:200ICC/IF: 1:50-1:200
Immunohistochemistry of paraffin-embedded human breast cancer using TMPRSS2 Rabbit pAb at dilution of 1:50 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human esophageal cancer using TMPRSS2 Rabbit pAb at dilution of 1:50 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Western blot analysis of extracts of various cell lines, using TMPRSS2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit .
Exposure time: 180s.
Immunofluorescence analysis of A-549 cells using TMPRSS2 Rabbit pAb at dilution of 1:50 . Blue: DAPI for nuclear staining.