Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Form:
Liquid
Application Dilute:
WB: 1:500-1:2000IHC: 1:50-1:200ICC/IF: 1:50-1:200
Immunohistochemistry of paraffin-embedded human breast cancer using TMPRSS2 Rabbit pAb at dilution of 1:50 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human esophageal cancer using TMPRSS2 Rabbit pAb at dilution of 1:50 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Western blot analysis of extracts of various cell lines, using TMPRSS2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit . Exposure time: 180s.
Immunofluorescence analysis of A-549 cells using TMPRSS2 Rabbit pAb at dilution of 1:50 . Blue: DAPI for nuclear staining.
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