PCNA Monoclonal Antibody, Biotin Conjugated, Clone: [2A1], Mouse

Produktübersicht

• Artikelname: PCNA Monoclonal Antibody, Biotin Conjugated, Clone: [2A1], Mouse
• Artikelnummer: BSS-BSM-10889M-BIOTIN
• Hersteller Artikelnummer: bsm-10889M-Biotin
• Alternativnummer: BSS-BSM-10889M-BIOTIN-100
• Hersteller: Bioss
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: IHC-Fr, IHC-P, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Konjugation: Biotin
• Alternative Synonym: Cyclin, DNA polymerase delta auxiliary protein, HGCN8729, MGC8367, Mutagen-sensitive 209 protein, Pcna/cyclin, PCNAR, Polymerase delta accessory protein, Proliferating Cell Nuclear Antigen, PCNA_HUMAN.

Produktbeschreibung

Auxiliary protein of DNA polymerase delta and epsilon, is involved in the control of eukaryotic DNA replication by increasing the polymerases processibility during elongation of the leading strand (PubMed:35585232).
Induces a robust stimulatory effect on the 3-5 exonuclease and 3-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902).
Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while Lys-63-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion (PubMed:24695737).

Produkteigenschaften

• Klonalität: Monoclonal
• Konzentration: 1ug/ul
• Klon-Bezeichnung: [2A1]
• NCBI: 5111
• Puffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
• Quelle: Recombinant human PCNA Protein
• Target-Kategorie: PCNA

Anwendungsbeschreibung

• Application Verdünnung: WB(1:300-5000), IHC-P(1:200-400), IHC-F(1:100-500)